Additional Selective and Differential Cell Culture Media
Promocell GmbH PromoCell Endothelial Cell Growth Medium 2 KIT 500 ml, Contains: C-22211, C-39211
Endothelial Cell Growth Medium 2 is a low-serum (2% V/V) medium that lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 µg / ml || Heparin | 22.5 μg / ml || Hydrocortisone | 0.2 µg / ml |For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Promocell GmbH PromoCell Airway Epithelial Cell Growth Medium KIT 500 ml, Contains: C-21260, C-39160
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Lifeline Cell Technology Cervical Epi Media Kit
ReproLife CX Medium Complete Kit. Culture medium for the growth of cervical epithelial cells. LM-0055 ReproLife CX Basal Medium and LS-1116 ReproLife CX LifeFactors Kit
Biolegend Recombinant Human E-Cadherin-F
Recombinant Human E-Cadherin-Fc Chimera (carrier-free); Reactivity: Human; Apps: BA; Size: 100 μg
NEOGEN Buffered Peptone Water (BPW) (ISO), 5 kg
Buffered Peptone Water (BPW) (ISO) is used for the non-selective pre-enrichment of a variety of microorganisms from food including Salmonella spp. and Cronobacter spp. from food. Buffered Peptone Water (BPW) (ISO) is not intended for use in the diagnosis of disease or other conditions in humans. Formulated to ISO 6579-1:2017, ISO 22694:2017, ISO 21528-1:2017 and ISO 12950:2010. This pre-enrichment medium is designed to help sublethally damaged salmonellae recover before introducing them into a selective medium. This nutrient medium is free from inhibitors and is well buffered to maintain pH 7.0 for the incubation period. Sublethal injury to salmonellae occurs in many food processes and this pre-enrichment step greatly increases recovery of these organisms. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water-iso
NEOGEN Columbia CNA Agar, 5 kg
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-cna-agar
MOLTOX Molecular Toxicology Inc Top Agar w/ L-Histidine & D-Biotin, 100 mL/bottle, Store at Room Temperature
For use with Minimal Glucose Agar and S. typhimurium strains in the Ames assay. MOLTOX provides all products necessary to conduct the Salmonella, E. coli, MOLTOX FT™, µAmes and UMU Mutagenicity Assays. All products are formulated and manufactured using the highest quality components and are consistent with the recommendations set forth by the historical literature corresponding to each test type. Certain Ames materials (e.g. STDiscs™ and Lyophilized S9) have been specifically developed in our laboratory for these assays. All products are thoroughly tested for performance in the assay and are accompanied by Quality Control and Formulation statements.
NEOGEN Nutrient Broth, 5 kg
Nutrient Broth is used for the cultivation of a wide variety of microorganisms. Nutrient Broth is not intended for use in the diagnosis of disease or other conditions in humans.In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Broth is the same formulation as Nutrient Agar, only Agar has been omitted.Nutrient Broth is used as a pre-enrichment medium when testing certain foods and dairy products for Salmonella spp. In dried or processed foods, salmonellae may be sub-lethally injured and in low numbers. The presence of other bacteria and food sample components may hinder growth and recovery of Salmonella spp.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-broth
NEOGEN YM Broth, 5 kg
YM Broth is used for the cultivation of fungi in a laboratory setting. YM Broth is not intended for use in the diagnosis of disease or other conditions in humans. YM Broth is prepared according to the formulation published by Wickerham. Wickerham suggested that YM Broth, acidified to pH 3.0 – 4.0, be used as an enrichment medium for yeasts from populations also containing bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days. This favors yeast recovery while preventing sporulation of molds. Media selectivity may be enhanced through acidification or addition of selective agents.For more information visit https://www.neogen.com/solutions/Microbiology/ym-broth
NEOGEN Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 10 kg
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic.For more information visit https://www.neogen.com/solutions/Microbiology/thiosulfate-citrate-bile-salts-sucrose-agar
NEOGEN Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar, 5 kg
Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios according to ISO 21872-1:2017 and ISO 21872-2:2007. Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar is not intended for use in the diagnosis of disease or other conditions in humans. TCBS Agar is also called Vibrio Selective Agar, and prepared according to the formula of Kobayashi et al. This formula is a modification of the selective medium from Nakanishi. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. This highly selective agar meets the nutritional requirements of Vibrio spp. Vibrio spp. are able to grow in media containing increased salt concentrations, and some species are halophilic.For more information visit https://www.neogen.com/solutions/Microbiology/thiosulfate-citrate-bile-salts-sucrose-agar
NEOGEN Yeast Extract Dextrose Chloramphenicol Agar, 5 kg
Yeast Extract Dextrose Chloramphenicol Agar is a selective medium for the enumeration of yeasts and molds in milk and other dairy products, and is not intended for use in the diagnosis of disease or other conditions in humans. The medium is said to have superior storage properties to O.G.Y.E. and also has the advantage of incorporating an autoclavable supplement.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-dextrose-chloramphenicol-agar
NEOGEN Phenylethanol Agar, 10 kg
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN m-FC Agar, 10 kg
m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans. Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment. Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.For more information visit https://www.neogen.com/solutions/Microbiology/m-fc-agar
ATHENA ES MEM with L-Glutamine
Minimum Essential Media (MEM), patterned after Eagle’s Media, is one of the most commonly used of all cell culture media and is well suited for the growth of a broad spectrum of mammalian cells. Compared to the earlier Basal Medium Eagle (BME), MEM is formulated with increased concentrations of amino acids. Because MEM media lacks proteins, lipids, or growth factors, it commonly requires supplementation with fetal bovine serum (FBS).